hepa 1-6  (ATCC)

Journal: Materials Today Bio

Article Title: A flaxseed oil body-based delivery system integrating calcium overload and lipid peroxidation for immunogenic cell death-driven immunotherapy

doi: 10.1016/j.mtbio.2026.103184

Figure Lengend Snippet: In vitro antitumor activity of Cur/FOBs@CaP. (A) Flow cytometric analysis of cellular uptake of Cur following different treatments. (B) Quantification of intracellular Cur fluorescence intensity in Hepa1-6 cells. (C) Cell viability of Hepa1-6 cells after various treatments. (D) Quantitative analysis of apoptotic cells, with total apoptosis defined as the sum of early and late apoptotic populations. (E) Flow cytometric analysis of apoptosis induced by different treatments. (F) Representative live/dead staining images of Hepa1-6 cells after different treatments (green, live cells; red, dead cells). (G) Quantitative analysis of cell death ratios under different treatments. (H) MDA levels in Hepa1-6 cells following different treatments. (I) Flow cytometric analysis of mitochondrial superoxide levels using MitoSOX staining. (J – K) Quantitative analysis (J) and representative fluorescence images (K) of intracellular ROS generation in Hepa1-6 cells following various treatments, visualized by DCFH-DA staining (green). (L) CLSM images showing changes in mitochondrial membrane potential (ΔΨm) assessed by JC-1 staining after different treatments. Red fluorescence indicates polarized mitochondria, whereas green fluorescence denotes mitochondrial depolarization. Nuclei were counterstained with Hoechst 33342. (M) Quantitative analysis of mitochondrial membrane potential (ΔΨm) based on JC-1 staining. (N) Representative confocal microscopy images of Rhod-2 AM fluorescence (red) showing intracellular Ca 2+ levels in Hepa1-6 cells after different treatments. (O) Quantitative analysis of relative intracellular Ca 2+ levels in Hepa1-6 cells following various treatments. All quantitative data are presented as mean ± SD (n = 3 independent experiments). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The mouse hepatocellular carcinoma Hepa1-6 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: In Vitro, Activity Assay, Fluorescence, Staining, Membrane, Confocal Microscopy

Journal: Materials Today Bio

Article Title: A flaxseed oil body-based delivery system integrating calcium overload and lipid peroxidation for immunogenic cell death-driven immunotherapy

doi: 10.1016/j.mtbio.2026.103184

Figure Lengend Snippet: In vitro induction of ICD and immunostimulatory effects by Cur/FOBs@CaP. (A) Scheme illustrating the mechanism by which ICD-induced DAMPs promote dendritic cell maturation and subsequently initiate antitumor immune responses. (B) Quantitative analysis of mean intracellular fluorescence intensity of CRT corresponding to (C). (C) Immunofluorescence staining showing CRT exposure in Hepa1-6 cells following different treatments. (D) Immunofluorescence images showing HMGB1 translocation and nuclear release in Hepa1-6 cells after different treatments. (E) Quantitative analysis of HMGB1 fluorescence intensity derived from (D). (F – G) Representative flow cytometric plots of matured DCs (CD11c + CD86 + CD80 + ) (F) and T cells (CD3 + CD4 + CD8 + ) (G) in vitro . (H – J) Quantification analysis of mature DCs (CD11c + CD86 + CD80 + ) (H) , CD3 + CD8 + T cells (I) and CD3 + CD4 + T cells (J) . (K) Levels of inflammatory cytokines (TNF-α and IFN-γ) measured in vitro . All quantitative data are presented as mean ± SD (n = 3 independent experiments).

Article Snippet: The mouse hepatocellular carcinoma Hepa1-6 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: In Vitro, Fluorescence, Immunofluorescence, Staining, Translocation Assay, Derivative Assay

Journal: Materials Today Bio

Article Title: A flaxseed oil body-based delivery system integrating calcium overload and lipid peroxidation for immunogenic cell death-driven immunotherapy

doi: 10.1016/j.mtbio.2026.103184

Figure Lengend Snippet: Transcriptomic profiling of Hepa1-6 cells following Cur/FOBs@CaP treatment. (A) Venn diagram of primary transcripts of Hepa1-6 cells between control groups and Cur/FOBs@CaP. (B – C) Volcano plot (B) and heat map (C) illustrating differentially expressed genes (DEGs) in Hepa1-6 cells after Cur/FOBs@CaP treatment. (D) GO enrichment analysis of DEGs in Cur/FOBs@CaP group. (E) KEGG pathway enrichment analysis of DEGs in Cur/FOBs@CaP group. (F – H) Western blot images (F) and corresponding quantification of GPX4 (G ) and p-CAMK II (H) expression in Hepa1-6 cells following the indicated treatments. Protein levels were normalized to β-actin. Data are presented as mean ± SD (n = 3). RNA-seq analysis was performed using biological replicates (n = 3 per group). Differential expression was defined based on standard thresholds (|log 2 fold change| ≥ 1 and adjusted p < 0.05).

Article Snippet: The mouse hepatocellular carcinoma Hepa1-6 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Control, Western Blot, Expressing, RNA Sequencing, Quantitative Proteomics